Research Article
Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate
Table 2
Purification of xylanase from P. glabrum.
| Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg protein) | Purification (fold) | Recovery (%) |
| Culture filtrate | 2571.50 | 28.61 | 89.84 | 1.00 | 100.00 | Supernatant 90% of (NH4)2SO4 | 2165.01 | 7.42 | 291.78 | 3.25 | 84.19 | Sephadex G-75 | 1978.10 | 4.32 | 457.89 | 5.10 | 76.92 |
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