αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors
Figure 3
Pluripotent marker expression in αVβ5 blocked hESCs and CD44 blocked hESCs. Immunoflourescent staining of pluripotent marker expression (Oct-3/4, Nanog, ALP, and SSEA-4) in hESCs, 24 hours after αVβ5 blockage and CD44 blockage when cultured in 2% O2 or 21% O2. Nuclei are counterstained with DAPI (indicated in blue). Scale bar = 100 μm.
