Delivery of RNA and Its Intracellular Translation into Protein Mediated by SDS-CTAB Vesicles: Potential Use in Nanobiotechnology

<table>Effect of freezing on the transfection efficiency. Vesicles were formed in the presence of mRNA CAT and kept frozen at −20°C for 24 hours. After thawing, the aggregates were treated with RNase and transfected into HEK-293 cells. Data clearly indicate that the RNase treatment almost abolishes the translation of CAT mRNA into protein. LIPO (Lipofectamine); <i>V</i>[25 <i>μ</i>M] (25 <i>μ</i>M vesicle concentration); <i>V</i>[50 <i>μ</i>M] (50 <i>μ</i>M vesicle concentration); <i>V</i>[100 <i>μ</i>M] (100 <i>μ</i>M vesicle concentration); CAT (naked CAT mRNA). </table>

BioMed Research International

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Figure 5

Figure 5: Delivery of RNA and Its Intracellular Translation into Protein Mediated by SDS-CTAB Vesicles: Potential Use in Nanobiotechnology 