Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages
Effects of low pH stress on LPS-stimulated cytokine production in rat alveolar macrophages (aMØs). Isolated aMØs were stimulated in vitro with either saline (control), LPS alone (1.5 μg/mL for 24 hrs), or LPS in combination with a preceding transient low pH stress injury (pH 1.75 for 1 min, Low pH + LPS). Cytokines produced were assessed by ELISA (CINC-1, MIP-2, MCP-1, and INF-) or cytotoxicity bioassay (TNF-). LPS versus control, LPS versus Low pH + LPS.
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