Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages
Intracellular Ca2+ concentration profile under various conditions, expressed as fluorescence excitation ratios (R 340/380, proportional to [Ca2+]i) in Fura-2 AM-loaded aMØs. (a) Isolated aMØs loaded with Fura-2 AM were exposed to low pH stress in vitro and fluorescence ratios recorded at the indicated time points following low pH injury. (b) Fura-2 AM-loaded aMØs were stimulated with either LPS alone (black bar) or LPS preceded by transient low pH stress injury (Low pH + LPS, gray bar). (c) Fura-2 AM-loaded aMØs were stimulated with either LTA alone (black bar) or LTA stimulation preceded by a transient low pH stress injury (Low pH + LTA, gray bar). versus corresponding baseline, presence of low pH stress versus absence of low pH stress.
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