Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages

Figure 4

Effects of intracellular Ca²⁺ depletion (BAPTA-AM pretreatment) on LPS-stimulated cytokine production in aMØs. Isolated aMØs were stimulated in vitro with BAPTA-AM (5 μM for 30 min), LPS (1.5 g/mL for 24 hrs), or LPS in combination with preceding BAPTA-AM (LPS + BAPTA-AM). Cytokines produced were assessed by ELISA (CINC-1, MIP-2, MCP-1, and INF-𝛽) or cytotoxicity bioassay (TNF-𝛼). ^#𝑃<0.05 LPS versus LPS + BAPTA AM.

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