Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages
Figure 8
Effect of acid aspiration (low pH stress) on bacterial clearance in vivo was assessed using a mouse model of pulmonary bacterial infection. Pulmonary bacterial challenge was initiated using intratracheal administration of known titers (starting inoculum) of E. coli (left panel) or S. pneumoniae (right panel). Acid aspiration was modelled using intratracheal administration of acidified saline (pH 1.25) prior to bacterial challenge (pulmonary bacterial challenge + low pH stress). Lung homogenates were prepared 24 hr after intratracheal bacterial challenge and bacterial load quantified. Bacterial pulmonary challenge alone versus bacterial pulmonary challenge + low pH stress. versus bacterial inoculation alone.
