Figure 1: Ventral midbrain showing dopamine neurons identified by tyrosine hydroxylase immunofluorescent labeling. Fresh-frozen brains were cryosectioned at 10 microns, acetone fixed, and immunolabeled in high salt buffer, prior to dehydration and delipidation in preparation for laser microdissection using a Zeiss LMD system. (a) Prior to microdissection. (b) Following selection and laser microdissection of several immunofluorescent dopamine neurons. Dashed outlines indicate where the dopamine cell bodies were laser-captured from. A number of labeled dopamine neurons remain for reference.