Optimization of Polygalacturonase Production from a Newly Isolated Thalassospira frigidphilosprofundus to Use in Pectin Hydrolysis: Statistical Approach

Table 6

Experimental design and results of PGase production using CCD.

Trial	𝜒1	𝜒2	𝜒3	𝜒6	^†Response (U/mL)	CCD Predicated (U/mL)
1	−1.00	−1.00	−1.00	−1.00	25.08±0.12	25.14
2	1.00	−1.00	−1.00	−1.00	23.11±0.15	22.83
3	−1.00	1.00	−1.00	−1.00	25.29±0.06	25.84
4	1.00	1.00	−1.00	−1.00	23.82±0.18	23.96
5	−1.00	−1.00	1.00	−1.00	20.91±0.21	20.93
6	1.00	−1.00	1.00	−1.00	23.25±0.06	22.50
7	−1.00	1.00	1.00	−1.00	23.62±0.12	23.70
8	1.00	1.00	1.00	−1.00	24.47±0.15	25.70
9	−1.00	−1.00	−1.00	1.00	24.32±0.18	23.66
10	1.00	−1.00	−1.00	1.00	21.36±0.09	20.92
11	−1.00	1.00	−1.00	1.00	20.87±0.08	20.29
12	1.00	1.00	−1.00	1.00	21.41±0.16	21.96
13	−1.00	−1.00	1.00	1.00	19.78±0.12	19.28
14	1.00	−1.00	1.00	1.00	28.08±0.25	28.41
15	−1.00	1.00	1.00	1.00	21.09±0.6	21.95
16	1.00	1.00	1.00	1.00	23.95±0.16	23.52
17	−2.00	0.00	0.00	0.00	18.88±0.09	18.59
18	2.00	0.00	0.00	0.00	17.76±0.14	17.84
19	0.00	−2.00	0.00	0.00	23.11±0.06	24.48
20	0.00	2.00	0.00	0.00	27.88±0.12	23.27
21	0.00	0.00	−2.00	0.00	24.56±0.14	24.51
22	0.00	0.00	2.00	0.00	21.02±0.13	21.86
23	0.00	0.00	0.00	−2.00	25.61±0.18	25.68
24	0.00	0.00	0.00	2.00	22.82±0.06	22.03
25	0.00	0.00	0.00	0.00	24.52±0.04	24.75
26	0.00	0.00	0.00	0.00	24.62±0.09	24.75
27	0.00	0.00	0.00	0.00	24.52±0.12	24.75
28	0.00	0.00	0.00	0.00	25.62±0.06	24.75
29	0.00	0.00	0.00	0.00	24.62±0.04	24.75
30	0.00	0.00	0.00	0.00	24.62±0.12	24.75

†Experimental values are mean of triplicates test.