Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
Figure 5
Identification of sFLT-1 mRNA from K562 and HL60 cells using RT-PCR. (a) GAPDH served as an internal RNA loading control. Lane M: DNA marker (1 Kb plus 100 bp, Mbiotech, Korea); lanes 1 and 2: K562 and HL60 cells transfected with pVAX1-sFLT-1, respectively; lanes 3 and 4: K562 and HL60 cells transfected with pVAX1-hMGFP. (b) Lane M: DNA marker (1 Kb plus 100 bp, Mbiotech, Korea); lanes 1 and 2: K562 and HL60 cells transfected with pVAX1-sFLT-1; lanes 3 and 4: K562 and HL60 cells transfected with pVAX1-hMGFP. Arrows indicate expression of GAPDH at 121 bp and sFLT-1 at 1000 bp.
