Digestibility and IgE-Binding of Glycosylated Codfish Parvalbumin

Figure 6

IgE reactivity of different forms of parvalbumin digested at various pHs. Panels show different pH applied during digestion, indicated in upper right corner of each panel. Lane 1: Native undigested; Lane 2: native digested for 1 minute; Lane 3: Native digested for 8 minutes; Lane 4: 5 hour glycosylated undigested; Lane 5: 5-hour glycosylated digested for 1 minute; Lane 6: 12-hour glycosylated undigested; Lane 7: 12-glycosylated digested for 1 minute; Lane 8: 12-hour glycosylated PV digested for 8 minutes. Inset upper left corner: Coomassie-stained SDS-PAGE of native parvalbumin (N) and marker proteins (M) used for immune-blot shown in (a)–(e). Molecular weights are indicated in kDa.