Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats

Table 2

Effect of saponarin and silymarin pretreatment on hepatic liver peroxidation and antioxidant profile in rats challenged with paracetamol.

Group	MDA^a	GSH^a	SOD^b	CAT^c	GPx^b	GR^b	GST^d
Control	0.655 ± 0.05	5.52 ± 0.63	0.169 ± 0.018	121.0 ± 7.8	0.131 ± 0.016	0.222 ± 0.028	0.233 ± 0.03
PC	0.854 ± 0.04*	0.96 ± 0.05**	0.114 ± 0.012*	88.3 ± 10*	0.089 ± 0.01*	0.137 ± 0.02*	0.160 ± 0.01*
SP	0.635 ± 0.07	5.14 ± 0.31	0.158 ± 0.015	110.4 ± 16	0.125 ± 0.02	0.207 ± 0.03	0.208 ± 0.02
PC + SP	0.704 ± 0.02^+	1.23 ± 0.15^*+	0.141 ± 0.02^*+	94.6 ± 7.8	0.108 ± 0.015	0.191 ± 0.02^+	0.194 ± 0.02^+
SL	0.673 ± 0.07	5.01 ± 0.64	0.162 ± 0.024	137.6 ± 13	0.144 ± 0.02	0.200 ± 0.09	0.245 ± 0.03
PC + SL	0.690 ± 0.06^+	1.40 ± 0.12^*+	0.138 ± 0.02^*+	108.1 ± 7^+	0.113 ± 0.01^+	0.199 ± 0.02^+	0.149 ± 0.03*

Treatment: paracetamol administered at a dose of 600 mg/kg i.p., alone (PC group) and two hours after the last administration of the hepatoprotective agents (SP + PC and SL + PC groups); saponarin (SP) (80 mg/kg bw/day) and silymarin (SL) (100 mg/kg bw/day were administered p.o. for 7 days. Data are expressed as mean ± SEM of six rats. *Significant difference from control values (Mann-Whitney U test, 𝑃<0.05). ⁺Significant difference from paracetamol-treated group (Mann- Whitney U test, 𝑃<0.05).
^anmol/g protein.
^bnmol/min/mg protein.
^cμmol H₂O₂/min/mg protein.
^dnmol CDNB-GSH conjugate formed/min/mg protein.