Figure 3: Detection of ALK fusion in lung cancer patient (LC14) by FISH, real-time RT-PCR, and immunohistochemistry. (a) FISH performed with Vysis LSI ALK Dual Color Break Apart FISH probes detected ALK fusion as split red and green signal. (b) An example results graph from real-time RT-PCR showing change in the normalized reporter signal (delta Rn) against PCR cycle number for reaction 1 of the AmoyDx EML4-ALK fusion gene detection kit. (c) and (d) Immunohistochemistry revealed strong expression of ALK in ALK-fusion positive patient (c) and no expression in ALK-fusion negative patient (d).