Molecular Imaging of Hepatocellular Carcinoma Xenografts with Epidermal Growth Factor Receptor Targeted Affibody Probes
Figure 2
Human hepatocellular carcinoma cell line characterization. (a) Assessment of EGFR expression in HCC cell lines, including Hep3B, PLC/PRF/5, and HepG2 cells. Protein ladders are indicated in kDa. Bands for EGFR and β-actin are indicated by arrowheads. (b) EGFR expression in HCC cell lines at mRNA level was assessed by quantitative real-time PCR. Relative quantification of EGFR RNA expression was validated by 18S RNA. (c) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 cells using FITC labeled anti-EGFR antibody. Positive staining of EGFR was shown in red. Nuclei staining using DAPI is shown in blue. (d) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 cells using Alexa680-. Staining using unlabeled Ac-Cys- as a blocking reagent was also shown. Positive staining of EGFR was shown in red. Nuclei staining using DAPI is shown in blue.
