Figure 3: Immunofluorescence and immunohistochemistry staining on subcutaneous HCC xenografts. (a) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 xenografts using Alex680- . (b) Immunohistochemistry (IHC) staining on paraffin-embedded subcutaneous tumor tissues, including Hep3B, PLC/PRF/5, and HepG2 tumors. Anti-EGFR antibody was used in 1 : 200 dilution. Nuclei staining was performed using Dako Cytomation Mayer’s hematoxylin histological staining reagent. (c) Representative photograph of PLC/PRF/5, Hep3B, and HepG2 subcutaneous tumors.