Figure 2: Light micrographs showing the localization of heparanase and heparan sulfate in palatal shelves of a mouse embryo at E14.5. (a) The palatal shelves were horizontal in apposition. (b) A higher magnification of the square marked in (a). Some epithelial cells at the tip of palatal shelves had heparanase labeling (arrowheads). Adjacent section to the section shown in (c). (c) HS labeling was diffuse and weak in the basement membrane at the tip of palate epithelium (arrowheads). Adjacent section to the section shown in (b). PS, palatal shelf; T, tongue; MB, maxillary bone; N; nasal septum. Bars: a, 100 μm; b, c, 25 μm.