Differential Influence of Components Resulting from Atmospheric-Pressure Plasma on Integrin Expression of Human HaCaT Keratinocytes

Figure 1

Cell number of recovered HaCaT cells (a) 24 h after treatment with surface DBD/air (red line; 𝑁=8) or DBD/argon (blue line; 𝑁=8) plasma for 20 s to 300 s and induction of apoptosis (b) after DBD/air treatment (▽=1 h after plasma treatment, ○ = 24 h after plasma treatment; 𝑁=8). Apoptosis was analysed by flow cytometry using Annexin V-FITC and PI (early apoptotoc cells = Annexin V⁺PI⁻). Untreated cells served as controls (see time 0). Results are given as mean ± SEM. ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001 (𝑡-test), ^#𝑃<0.05 (Mann-Whitney test) versus control cells; ^⋆𝑃<0.05, ^⋆⋆𝑃<0.01 (𝑡-test); ^∘𝑃<0.05 (Mann-Whitney test).

(a)

(b)