Moscatilin Inhibits Lung Cancer Cell Motility and Invasion via Suppression of Endogenous Reactive Oxygen Species

Figure 3

Effects of moscatilin on H23 cell migration. (a) Confluent monolayer of H23 cells was wounded using a 1 mm width tip and incubated with nontoxic dose of moscatilin (0-1 μM) for 24 h. Wound space was analyzed and represented as migration level relatively to the change of those in nontreated cells. Data represent the means ± SD (𝑛=4). ^∗𝑃<0.05 versus nontreated control cells. (b) Confluent monolayer of H23 cells was wounded using a 1 mm width tip and incubated with moscatilin (1 μM) or without for various times (12–48 h). Wound space was analyzed and represented as migration level relatively to the change of those in nontreated cells. ^∗𝑃<0.05 versus nontreated control cells. (c) After indicated treatment, migrating cells in the denuded zone were photographed. (d) H23 cell migration was examined by transwell assay for 24 h. Data were plotted as an average number of cells in each field and represented the means ± SD (𝑛=4). ^∗𝑃<0.05 versus nontreated control cells. (e) Cells were treated with moscatilin (1 μM) or without for various times (12–48 h) on transwell. Data were plotted as an average number of cells in each field and represented the means ± SD (𝑛=4). ^∗𝑃<0.05 versus nontreated control cells. (f) Migratory cells at the basolateral side of membrane were stained with Hoechst33342 for 30 min and visualized under fluorescence microscopy.