Engineering Microbial Cells for the Biosynthesis of Natural Compounds of Pharmaceutical Significance

Figure 3

Pathway for in vivo production of taxadiene and 5-α-hydroxytaxadiene in the route to taxol via the methylerythritol phosphate pathway in E. coli (adapted from [23]). Genes dxs, idi, ispD, and ispF, respectively, encoding for 1-deoxy-D-xylulose-5-phosphate synthase, isopentenyl diphosphate isomerase, isoprenoid synthase domain, and 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase. IPP: isopentenyl diphosphate; DMAPP: dimethylallyl diphosphate; GPP: geranyl diphosphate; FPP: farnesyl diphosphate; GGPP: geranylgeranyl diphosphate.