Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
An overview of biomarker discovery using tissue culture. Cancer cells are cultured in plates. The CM as well as cells is collected separately. Extracted proteins from each fraction are processed for either in-gel or in-solution digestion followed by the detection of peptides by mass spectrometric approach. Data analysis leads to detection of candidate biomarkers.