Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
(a) A schematic representation of ESI-MS—solvent along with sample flows from the needle with electrical potential generating charged droplets. The droplets carry the sample, and they are desolvated by applying heat and nebuliser gas to produce ions. These ions are now separated according to m/z ratio in the mass analyzer and registered by detector. (b) A schematic representation of MALDI-MS—the sample is mixed with the matrix and allowed to crystalize on the MALDI plate, when the laser hits the sample-matrix mixture on the plate, matrix absorbs the energy of the laser to get vaporized along with samples. Next, the charge exchange takes place from matrix and sample ions are generated.