The Protective Effect of Silybin against Lasalocid Cytotoxic Exposure on Chicken and Rat Cell Lines

Table 1

Effective concentration, EC₅₀ (μM) of lasalocid, silybin, and lasalocid EC₅₀ in the interaction with silybin 1–250 μM range concentrations estimated by the MTT, CBB, and LDH assays on chicken hepatoma (LMH) and rat myoblasts (L6) cell line, mean ± SEM, (𝑛=3).

	Lasalocid		Silybin		Silybin with lasalocid (~EC50)
	LMH	L6	LMH	L6	LMH	L6
MTT	7.0 ± 0.54^a	14.0 ± 1.08^b	70.4 ± 1.05^a	247 ± 3.90^c	114 ± 11.37^a	N/D
CBB	6.8 ± 0.55^a	20.9 ± 1.36^c	93.4 ± 2.63^b	104 ± 6.50^d	154 ± 9.22^b	N/D
LDH	8.1 ± 0.54^a	7.6 ± 1.39^a	93.6 ± 0.98^b	70.5 ± 9.51^a	157 ± 6.65^b	42.7 ± 1.15^c

The different superscripts (a, b, c, d) within a column of compounds indicate significant differences between the methods (𝑃<0.05), N/D: not detected within the studied concentration range.