BioMed Research International / 2013 / Article / Fig 3

Research Article

α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli

Figure 3

ICDH’s tellurite reductase activity. (a) TR activity determined by plaque assay. Controls are (1) only buffer, (2) buffer + 1 mM β-mercapto ethanol (β-ME), (3) buffer + β-ME + 1 mM tellurite, and (4) buffer + βME + tellurite + bovine serum albumin (BSA). Purified ICDH was added at 5, 10, 25, 50, and 100 μg, as indicated in the figure. (b) TR activity was revealed after native polyacrylamide gel electrophoresis as described in methods. A representative gel is shown. BSA and purified dihydrolipoyl dehydrogenase (E3) from Aeromonas caviae were used as negative and positive controls, respectively.

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.