α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli
ICDH’s tellurite reductase activity. (a) TR activity determined by plaque assay. Controls are (1) only buffer, (2) buffer + 1 mM β-mercapto ethanol (β-ME), (3) buffer + β-ME + 1 mM tellurite, and (4) buffer + βME + tellurite + bovine serum albumin (BSA). Purified ICDH was added at 5, 10, 25, 50, and 100 μg, as indicated in the figure. (b) TR activity was revealed after native polyacrylamide gel electrophoresis as described in methods. A representative gel is shown. BSA and purified dihydrolipoyl dehydrogenase (E3) from Aeromonas caviae were used as negative and positive controls, respectively.
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