Mechanisms by Which Interleukin-6 Attenuates Cell Invasion and Tumorigenesis in Human Bladder Carcinoma Cells
Figure 5
IL6 attenuates tumorigenesis of bladder carcinoma cells. (a) Nude mice were inoculated subcutaneously with HT-DNA (black circle) or HT-IL6 (white circle) cells. (b) Nude mice were inoculated subcutaneously with T24-GFPsi (black circle) or T24-IL6si (white circle) cells. Tumor size was measured with vernier calipers and the data are presented as tumor size in mm3 (±SE) at indicated time intervals . Blood samples were collected from experimental animals by cardiocentesis and were assayed for IL6 (c, d) levels by ELISA. Data are presented as the mean (±SE) of the IL6 protein levels. The expression of the MASPIN, NDRG1, and KAI1 genes in the tumor tissues () of (e) the HT-DNA (black bars) and HT-IL6 (white bars) xenograft groups and (f) the T24-GFPsi (black bars) and the T24-IL6si (white bars) xenograft groups was determined using RT-qPCR. Data are presented as mean fold induction of the mRNA levels of the target gene (±SE) relative to the mock-transfected xenograft groups .
