A comparison between the most used methods applied to metabolomic research.
|HPLC-MS||a, b, c||High sensitivity, nonvolatile metabolites, expensive||[12, 13]|
|GC-MS||a, c||High sensitivity, only volatile compounds, nonexpensive||[14, 15]|
|NMR||b, d, e||For highly abundant metabolites, nondestructive, very reproducible, low sensitivity|||
|MS||a, b, c, d, e||High sensitivity, usually coupled with other techniques|||
|Raman spectroscopy||e||Low resolution, sensitivity, automated, high throughput|||
|Ref: a: metabolite target analysis; b: metabolite profiling; c: metabolomics; d: metabolite flux analysis; e: metabolic fingerprinting. MS: mass spectrometry; HPLC-MS: high-performance liquid chromatography-MS; GS-MS: gas chromatography-MS; NMR: nuclear magnetic resonance spectroscopy.|