Figure 2: Estimation of endogenous expression of FVIII in untransfected cells. Fixed and permeabilized cells were incubated with anti-FVIII monoclonal antibodies ab41188 and ESH8. Binding of FVIII protein was detected using Alexa Fluor 488 labeled goat anti-mouse IgG secondary antibodies. Each histogram depicts the fluorescence intensity of 10,000 cells stained with isotype control (blue), IgG2a ab41188 (red), and ESH8 (orange). Figures depict the ICS staining in (a) mouse fibroblasts (NIH-3T3), (b) Chinese hamster ovary, (CHO) and (c) human embryonic kidney (HEK-293) cells.