Research Article

Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry

Figure 5

No shift in fluorescence intensity in unpermeabilized PBMCs. Representative histogram showing flow cytometry analysis of human unpermeabilized PBMCs stained with monoclonal antibodies ESH8 (orange) and ab41188 (red). Minimal shift in the fluorescence intensity of the anti-FVIII antibodies was observed compared to the isotype control (blue).
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