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BioMed Research International
Volume 2013 (2013), Article ID 795095, 8 pages
http://dx.doi.org/10.1155/2013/795095
Research Article

Low-Cytotoxic Synthetic Bromorutaecarpine Exhibits Anti-Inflammation and Activation of Transient Receptor Potential Vanilloid Type 1 Activities

1Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, No. 250, Wu-xing Street, Taipei 110, Taiwan
2Institute of Chemical Engineering, College of Engineering, National Taipei University of Technology, Taipei, Taiwan
3Department of Food and Beverage Management, Taipei College of Maritime Technology, Taipei, Taiwan
4Department of Internal Medicine, School of Medicine, Taipei Medical University, No. 250, Wu-xing Street, Taipei 110, Taiwan
5Department of Biochemistry, College of Medicine, Taipei Medical University, No. 250, Wu-xing Street, Taipei 110, Taiwan
6Orthopedics Research Center, Taipei Medical University Hospital, Taipei, Taiwan
7Institute of Biochemical and Biomedical Engineering, College of Engineering, National Taipei University of Technology, Taipei, Taiwan

Received 12 September 2013; Accepted 29 October 2013

Academic Editor: Joen-Rong Sheu

Copyright © 2013 Chi-Ming Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Br-RUT was Designed and synthesized according to the Scheme 1. Fig. S1 is the FT-IR spectrum of Br-RUT alone with its chemical structure as shown in the insert (Left). The two signature IR bands of 1668 cm-1and 3330 cm-1 reflect the corresponding functional groups, the carbonyl and the N-H stretching, respectfully. Fig. S2 is the 1H NMR spectrum of Br-RUT in D6-DMSO alone with its chemical structure as shown in the insert. The protons of the chemical structure has been assigned with alphabetical letters (a through i), and each letter corresponds to the assignments of the peaks within spectrum.

Fig. S3 is the effects of Br-RUT on TRPV-1 expression and eNOS phosphorylation in human coronary artery endothelial cells (HCAECs). To validate the expression of TRPV1 in the endothelium, the TRPV1 protein of HCAECs was detected by immunoblotting. Br-RUT treatment (0-10 μM) for 24 h increased TRPV1 protein amounts compared to the control group after normalization with α-tubulin levels. Br-RUT treatment (0-10 μM) for 24 h increased eNOS phosphorylation compared to the unphosphorylated eNOS (lower panel).

  1. Supplementary Material