Figure 1: Silencing of RT1-A expression in the LEWis (LEW) rat-derived fibrosarcoma cell line. The fibrosarcoma cell line was transduced using either lentiviral vectors encoding for nonspecific short hairpin RNA (shNS) or a β2-microglobulin (β2m-) specific short hairpin RNA (RN_shβ2m). (a) Relative transcript levels of β2m in fibrosarcoma cells, as determined by real-time PCR 4 days after transduction. (b) Flow cytometry analysis of RT1-A expression on nontransduced fibrosarcoma cells and after the delivery of shNS or RN_shβ2m.