A Lys49 Phospholipase , Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region
Figure 5
Signaling pathways involved in MT-II-induced LD formation. Peritoneal macrophages were incubated with one of the following: (a) PKC inhibitor H7 Dihidro (6 μM) for 1 h or the PI3 K inhibitor LY294002 (1 μM) for 1 h; (b) the p38MAPK or ERK1/2 inhibitors SB202190 (1 μM) or PD98059 (25 μM) for 1 h before stimulation with MT-II (0.8 μM) for 1 h. LDs were counted using light microscopy after osmium staining. Each bar represents the mean ± SEM of the number of LDs/cell in 50 counted cells. Values represent means ± SEM from three to five animals. * compared with control cells; # compared with MT-II-stimulated cells.
