A Lys49 Phospholipase , Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region
MT-II induces upregulation of PLIN2 expression in macrophages. Peritoneal macrophages were incubated with MT-II (0.8 μM) or RPMI (Control) for 3, 6, and 12 h. (a) Western blotting of PLIN2 and β-actin (loading control) in macrophage extracts. (b) Densitometric analysis of the band intensities of immunoreactive PLIN2. The densities (in arbitrary units) were normalized with those of -actin. Results are expressed as mean ± S.E.M. from three experiments. * compared with controls.
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