A Lys49 Phospholipase , Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region

PLIN2 and LD colocalize in macrophages stimulated by MT-II. Macrophages incubated with RPMI (control) or MT-II (0.8 μM) for 3 h were labeled for LDs (fluorescent Nile Red) and for PLIN2 (FITC-conjugated immunocomplex). Merged image shows colocalization of PLIN2 to LDs. Cell nuclei are observed by DIC. The pictures are representative of three independent experiments.