BioMed Research International / 2013 / Article / Fig 8

Research Article

A Lys49 Phospholipase , Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region

Figure 8

Effects of inhibitors of c cPLA2 and iPLA2 on MT-II induced LDs formation. Peritoneal macrophages were incubated with Pyr-2 (1 μM) or BEL (2 μM) compounds for 30 min and then with MT-II (0.8 μM) for 3 h. LDs were quantified using light microscopy after osmium staining. Each bar represents the mean ± sem LDs/cell in 50 counted cells. Values represent means ± SEM from 3–5 animals. * compared with control group; # compared with MT-II-stimulated cells.

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