BioMed Research International / 2013 / Article / Fig 4

Research Article

Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents

Figure 4

Loss of mitochondrial membrane potential ( ) in P. hysterophorus leaf ET extract treated HL-60 cells. (a) Untreated cells (Control), (b) treated cells, and (c) positive control. HL-60 cells (1 × 106/mL/well) were incubated with indicated doses of ethanol (ET) extract and camptothecin for 24 h. Cells were stained with Rhodamine-123 (200 nM) for 1 h and analyzed in FL-1 versus FL-2 channels of flow cytometer as described in materials and methods section. Data are representative of one of three similar experiments.
(a) Untreated cells
(b) ET 100 μg/mL
(c) Camptothecin 5 μM

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