An In Vitro Culture System for Long-Term Expansion of Epithelial and Mesenchymal Salivary Gland Cells: Role of TGF-1 in Salivary Gland Epithelial and Mesenchymal Differentiation
Figure 2
Histology of Col1a1-GFP derived submandibular salivary gland demonstrates salivary gland mesenchymal markers. (a) and (b), smooth muscle actin (SMA) (in red) staining for myoepithelial cells surrounding acini and ductal structures demonstrated that SMA-positive cells were located closely to, but did not colocalized with, GFP-positive cells. (c) and (d), collagen type I (in red) stained the extracellular matrix in salivary glands, and also colocalized with GFP-positive cells, confirming that GFP expression was driven by the procollagen type I promoter. AC = salivary gland acini, D = salivary gland duct, DE = ductal epithelium. Arrowheads indicate salivary gland ducts (D) with lumens. Scale bars = 100 μm.
