An In Vitro Culture System for Long-Term Expansion of Epithelial and Mesenchymal Salivary Gland Cells: Role of TGF-𝛽1 in Salivary Gland Epithelial and Mesenchymal Differentiation

Table 1

Mouse-specific primer sequences.

Gene	Forward primer (5′→3′)	Reverse primer (5′→3′)	GenBank accession number
Gapdh (R)	CTCGTCCCGTAGACAAAATGG	CGCTCCTGGAAGATGGTG	NM_008084
Gapdh (Q)	GGGAAGCCCATCACCATCT	GCCTCACCCCATTTGATGTT	NM_008084
Amylase-1(Amy-1) (B)	GGTGCAACAATGTTGGTGTC	ACTGCTTTGTCCAGCTTGAG	NM_007446
Aqp-5 (B)	CGACCGTGTGGCTGTGGTCA	GTGCCGGTCAGTGTGCCGTC	NM_009701
Collagen type I (B)	ACGGCTGCACGAGTCACAC	GGCAGGCGGGAGGTCTT	NM_007742
Fgf-7 (R)	ACTGTTCCAGCCCCGAGCGA	TTCCCCTCCGCTGTGTGTCCA	NM_008008
Fgf-7 (Q)	GTCCGGAGCAAACGGCTACGA	TGTGTCGCTCGGGGCTGGAA	NM_008008
Fgf-10 (R)	CGCAGAGGGGCGCAGATGTC	GCCTGTCCTCGCTCCGTCCT	NM_008002
Fgf-10 (Q)	TGGTGTCACAGGAGGCCACCAA	CGCACATGCCTTCCCGCACT	NM_008002
Pdgfr-a (R)	TTTGTGCCTCTCGGGATGA	TGACGGGCAGCACATTCA	NM_011058
Occludin (Q)	AGACCCAAGAGCAGCCAAAG	GGAAGCGATGAAGCAGAAGG	NM008756
Tgf-b1 (Q)	CTACTATGCTAAAGAGGTCACC	TTTCTCATAGATGGCGTTGTTGC	NM_011577
Tgf-br1 (Q)	GGAAATTGCTCGACGCTGTT	TTCTCATTTCTTCAACCGATGGA	NM_009370
ZO-1 (Q)	CGAGGCATCATCCCAAATAAGAAC	TCCAGAAGTCTGCCCGATCAC	NM_009386

Note: (R): primer sequences used for RT-PCR; (Q): primer sequences used for Q-RT-PCR; (B): primer sequences used for both RT-PCR and Q-RT-PCR.