Figure 5: Effects of Spirogyra neglecta extract on insulin-stimulated rOat3 substrate transports. Rat renal cortical slices were preincubated for 30 min in the presence or absence of 30 μg/mL insulin and followed by incubation with organic anion substrates. (a) 50 nM of [3H]ES or (b) 5 μM of [3H]PAH for 30 min. Data are expressed as fold over control (without insulin). Each experiment was performed from separate animals and at least 5 renal slices were used in each condition ( ). indicates significant differences from the slices incubated with buffer alone.