Figure 7: Effect of Spirogyra neglecta extract on the expressions of stress-sensitive genes. (a) mRNA expression levels of catalase (CAT), glutathione peroxidase (GPx), and copper-zinc superoxide dismutase (Cu-ZnSOD) from ND, ND + SNE1.0, DM, DM + SNE1.0, and DM + vitamin C  rat kidneys. Total RNA was extracted from rat cortical tissues and mRNA levels were measured using quantitative real-time PCR (qPCR). The results are expressed as mean ± S.E.M ( ). (b) p65NFκB expression in subcellular fractions of rat kidneys. Whole cell lysate, cytosolic, and nuclei fractions were extracted from rat renal cortical tissues. The samples were then separated using electrophoresis and western blotting. Anti-p65NFκB antibody was subsequently detected while anti-lamin B1 and anti-β-actin antibodies were used as a nuclei marker and loading control, respectively. The data are expressed as mean ± S.E.M. and repeated from separate sets of animals ( ). A representative blot of p65 NFκB protein expression is shown in (top (b)) and quantification of relative protein expression in each fraction is presented in (bottom (b)). indicates the significant differences from normal (ND) and indicates the significant differences from T2DM (DM) rats.