Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.

Table 1

Number, frequencies of abnormal PMCs in 1st and 2nd meiotic divisions, frequencies of meiotic abnormalities, types of abnormal meiotic PMCs, and percentages of fertile PGs after irradiation of Vicia faba seedlings by various irradiation doses of FN and UV-B.

Irradiation types	Doses	1st meiotic; division		2nd meiotic; Division		Total no. of PMCs	Total no. of abnormal PMCs	% of abnormal PMCs ± SE	Types and frequency of meiotic abnormalities of PMCs								PGs
		Total no. of PMCs	% of abnormal Cells	Total no. of PMCs	% of abnormal Cells				Stickiness	Disturbed	Unorient.	Micronuclei + fragments	Lagging + free	Bridge	Tripolar	Multipolar	No. of PGs	Mean % of fertility
Un- irradiated	0.00	835	0.00	1050	0.00	1885	0	0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	600	99.98±0.12
FN fluencies (Φ) n/cm²	2.5 × 10^5	541	38.26	339	47.79	880	369	38.80±0.54	19.56	30.37	14.43	13.90	8.52	2.64	9.13	1.60	600	88.90±1.40
	2.3 × 10^6	446	15.47	505	14.85	951	145	14.23±0.95	25.06	37.95	12.51	5.82	6.71	6.26	3.58	1.12	600	99.96±0.12
	3.0 × 10^7	515	58.64	440	42.50	955	498	52.15±2.17	25.56	27.37	10.30	13.73	4.61	8.40	7.59	2.44	600	65.10±0.06
	1.5 × 10^8	412	66.50	336	65.18	748	493	65.91±1.00	41.92	12.47	11.86	15.75	6.95	3.48	4.94	2.69	600	35.88±0.12
UV-B doses; μw/cm²	1/2 h	526	33.08	435	38.39	955	341	35.71±0.79	12.00	7.84	20.85	42.22	3.82	10.04	0.88	2.35	600	81.99±0.02
	1 h	459	21.70	435	19.06	1158	236	20.38±0.54	22.40	5.91	8.93	33.20	4.48	19.55	3.26	3.26	600	96.72±0.25
	2 h	424	49.06	450	43.55	874	404	46.22±1.12	31.68	4.70	8.17	25.57	6.44	20.96	1.49	1.00	600	57.77±0.23
	3 h	680	49.71	421	49.64	1101	607	55.13±0.18	37.50	8.25	10.51	31.16	3.44	6.34	1.27	1.74	600	25.54±0.26