Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.

Table 4

RAPD-PCR amplification products of DNA extracted from seeds yielded from irradiated Vicia faba by FN and UV-B using six random primers.

			Total no. of scorable bands							Polymorphic bands
Primers	Sequence of primers from 5′→3′	Amplicon lengths (pb)	Fluencies of fast neutrons radiation (n/cm²)		Control	Various doses of UV-B radiation (μw/cm²)		Total no.	% of total bands	No. of unique bands	% of unique bands	No. of nonunique bands	% of nonunique bands	% of polymorphic bands	No. of monomorphic bands	% of monomorphic	% of Total polymorphism
			1.5 × 10^8	2.3 × 10^6		1 h	3 h
			Lane 1	Lane 2	Lane 3	Lane 4	Lane 5
A-02	TGCCGAGCTG	1500–50	12	13	10	10	12	57	22.98	4	7.02	9	15.79	17.54	4	7.02	58.82
A-03	AGTCAGCCAC	1300–50	11	15	13	11	10	61	24.60	6	9.83	15	24.59	34.43			100.00
A-10	GTGATCGCAG	550–50	6	5	4	9	6	30	12.10	3	10.00	4	13.33	20.00	1	3.33	75.00
A-12	TCGGCGATAG	900–65	5	8	7	7	9	35	14.11	4	11.43	7	20.00	31.43	1	2.86	91.67
A-15	TTCCGAACCC	700–75	8	8	7	6	5	34	13.71	5	14.71	5	14.71	23.53	1	2.94	72.73
A-17	GACCGCTTGT	800–65	5	11	5	7	3	31	12.50	5	16.13	9	29.03	45.16	1	3.23	93.33
	Overall total DNA bands; %Total DNA bands		47; 18.95	60; 24.19	46; 18.55	50; 20.16	45; 18.15	248		27	10.89	49	19.78	30.65	8	3.23	90.80