The Cytotoxic Effect of Magainin II on the MDA-MB-231 and M14K Tumour Cell Lines

Table 4

The percentages of living, apoptotic, and dead mesothelioma cells were determined by flow cytometry, as shown in this table, where Q1 quadrant includes dead cells (7-AAD positive), Q2 quadrant contains late apoptotic (7-AAD positive, AnnexinV positive), and Q4 quadrant contains early apoptotic cells (AnnexinV positive, 7-AAD negative). Viable cells (7-AAD negative, AnnexinV negative) were enclosed in the Q3 quadrant.

M14K; 5000 events/well	Q1 (7-AAD+)	Q2 (7-AAD+ Annexin V+)	Q3 (7-AAD− Annexin V−)	Q4 (Annexin V+ 7AAD−)
	%	%	%	%
Negative control (NC)	2.1	3.8	89.6	4.5
Magainin II 120 μM (M120)	2.8	47.8	35.9	13.5
Magainin II 60 μM (M60)	0.3	8.6	85.2	5.9