836429.fig.004a
(a)
836429.fig.004b
(b)
Figure 4: Purified recombinant N-APP by Sephacryl S-200 high resolution chromatography. (a) Profile of gel filtration of the fractions from the Q Sepharose fast flow chromatography. The arrow indicates the purified protein eluting peak (13.3 mL) which corresponds to the 6th, 7th, 8th, 9th, and 10th collection tube; (b) SDS-PAGE analysis of the purified N-APP after gel filtration. M: protein marker; lanes 6–10: the eluting fraction which corresponds to the 6th, 7th, 8th, 9th, and 10th collection tube.