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BioMed Research International
Volume 2013, Article ID 842192, 8 pages
Research Article

Osteogenic Matrix Cell Sheet Transplantation Enhances Early Tendon Graft to Bone Tunnel Healing in Rabbits

1Department of Orthopaedic Surgery, Nara Medical University, Shijocho 840, Kashihara, Nara 634-8522, Japan
2Department of Public Health, Health Management and Policy, Nara Medical University, Shijocho 840, Kashihara, Nara 634-8522, Japan
3Department of Biomedical Engineering, Doshisha University, Tataramiyakodani 1-3, Kyotanabe, Kyoto 610-0394, Japan
4Department of Artificial Joint and Regenerative Medicine for Bone and Cartilage, Nara Medical University, Shijocho 840, Kashihara, Nara 634-8522, Japan

Received 24 April 2013; Revised 3 July 2013; Accepted 13 August 2013

Academic Editor: Chunfeng Zhao

Copyright © 2013 Yusuke Inagaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The objective of this study was to determine whether osteogenic matrix cell sheets (OMCS) could induce bone formation around grafted tendons, thereby enhancing early stage tendon to bone tunnel healing in skeletally mature male Japanese white rabbits. First, the osteogenic potential of rabbit OMCS was evaluated. Then, the OMCS were transplanted into the interface between the grafted tendon and the bone tunnel created at the tibia. Histological assessments and biomechanical tensile testing were performed after 3 weeks. The rabbit OMCS showed high alkaline phosphatase (ALP) activity, positive staining of ALP, and osteogenic potential when transplanted subcutaneously with beta tricalcium phosphate disks. Newly formed bony walls and positive collagen type I staining were seen around the grafted tendon with OMCS transplantation, whereas such bony walls were thinner or less frequent without OMCS transplantation. Micro-computed tomography images showed significantly higher bone volume in the OMCS transplantation group. The pullout strength was significantly higher with OMCS (  N/mm2) than without OMCS (  N/mm2). These results show that OMCS enhance early tendon to bone tunnel healing. This method can be applied to cases requiring early tendon to bone tunnel healing after ligament reconstruction surgery.