BioMed Research International / 2013 / Article / Fig 1

Research Article

A New Dual-Promoter System for Cardiomyocyte-Specific Conditional Induction of Apoptosis

Figure 1

Schematic representation of the parental lentiviral backbone (a), the subsequent cloning intermediate (b), and the final dual-promoter lentiviral vector used in the study (c). A detailed description of backbone components is available elsewhere [15]. The intermediate was derived from the parental clone by removing CMV and GPF; CMV was replaced with the cardiac-specific promoter (NCX1) and rtTA; GFP was replaced by the Tet-responsive element cassette (TRE) and AIFsh. The resulting pTY2-NCX1-rtTA-TRE-AIFsh construct has been named pNRTA for shortness. CMV: cytomegalovirus promoter; GFP, green fluorescent protein; MCS: multicloning site; fNCX1: feline Na+-Ca2+ exchanger promoter; rtTA-reverse repressor of TRE; TRE, tet-responsive element; Tet: tetracycline; AIFsh: apoptosis-inducing factor-short.