Figure 4: Effect of rotenone on intracellular localization of MEF2D. (a) Representative Western blot analysis of MEF2D in the cytosolic (C) and nuclear (N) fractions obtained from SH-SY5Y cells in basal conditions and after exposure to 100 and 200 nM rotenone for 24 hours. The immunoreactivities of beta-actin, used as internal standard for the cytosol, and PARP, used as internal standard for the nuclei, were also shown. (b) Mean values of MEF2D protein expression in cytosolic and nuclear fractions, expressed as ratio to beta-actin and PARP, respectively. , repeated measures ANOVA test, followed by Dunnett’s post-test; ; versus vehicle.