[Retracted] MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP
Figure 3
Knockdown of iASPP or overexpression of miR-124 inhibits iASPP expression and increases NF-κB(p65) expression. SW480 or HT29 or HT29 cells were infected with scrambled miR lentivirus (without miR-124) (miR-SRC), miR-124 lentivirus, iASPP shRNA, or control shRNA, lentivirus. (a) Real-time PCR analysis of miR-124 and U6 expression. (b) Real-time PCR quantification of iASPP mRNA expression; compared to SW480 or HT29 or HT29 cells transfected with scrambled miR-124 lentivirus (miR-SRC) or control shRNA lentivirus. (c) Western blot of iASPP protein expression. Expression of iASPP was inhibited by both iASPP shRNA and miR-124, compared to SW480 or HT29 or HT29 cells expressing Con-shRNA or miR-SCR. (d) Western blot of NF-κB(p65) protein expression. Expression of NF-κB(p65) was increased by both iASPP shRNA and miR-124, compared to SW480 or HT29 or HT29 cells expressing Con-shRNA or miR-SCR. The figure is representative of three experiments with similar results.
