[Retracted] MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP

Figure 5

Overexpression of iASPP inhibits the effects of miR-124 on iASPP and NF-κB(p65) expression. SW480 or HT29 cells stably expressing miR-SCR or miR-124 were transfected with iASPP ORF-expressing plasmid. After 48 h, the levels of specific proteins were analyzed by immunoblotting, and the band intensities were quantified using Image-Pro Plus and normalized to β-actin. (a and c) Overexpression of iASPP increased iASPP protein expression. (b and d) Overexpression of iASPP decreased NF-κB(p65) protein expression; ^∗∗𝑃<0.01.

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