Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of <i>Clostridium difficile</i> from Stool Samples of the Hospitalized Patients

<table>Agarose gel electrophoresis of the PCR products tested with five primer pairs using 20 ng of genomic DNA of <i>C. difficile</i> R20291 as template. Each primer pair was tested individually and in combination with all 5 primer pairs in the multiplex PCR. Lane M: 100-bp DNA ladder marker. Lanes 1–5, single-plex PCR reactions using primers specific to <i>tcdA</i>, <i>tcdB</i>, <i>cdtA</i>, <i>cdtB</i>, and <i>16s rDNA</i>, respectively. Lane 6: multiplex PCR with all four toxin-specific primers and 16S rDNA primers.</table>

BioMed Research International

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Figure 1

Figure 1: Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of <i>Clostridium difficile</i> from Stool Samples of the Hospitalized Patients 

Figure 1 | Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients 