BioMed Research International / 2013 / Article / Fig 1

Research Article

Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

Figure 1

Midgut total protein extraction with Triton X-100 and VOPBA. (a) Proteins were extracted from mosquito MG tissue at different Triton X-100 concentrations, separated by SDS-PAGE, and stained with Coomasie Blue. Triton X-100 concentrations were 0.01, 0.05, 0.1, 0.5, and 1% corresponding to lane 1 to 5, respectively. (b) Proteins, separated by SDS-PAGE, were blotted onto PVDF and incubated with biotinylated DENV-2 and then with AP-Streptavidin. Proteins recognized by DENV-2 were developed with BCIP/NBT according to the procedure previously described [8]. The apparent molecular weights of these proteins are shown on the right side of (b). Molecular weight markers are shown on the left side in (a) and (b).

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.