Influenza Virus Specific CD8⁺ T Cells Exacerbate Infection Following High Dose Influenza Challenge of Aged Mice

Table 1

Experimental study design.

Groups	Groups of mice; (nos. of mice)	Prime	Analyses after the prime (compartment)	Boost (time after prime)	Analyses after the boost (compartment)	Challenge (time after prime)	Time of analyses after challenge (compartment)
Experiment 1 (Figures 1(a) and 1(b))
Group 1	Young (15)	AdC68NP	Wks 2, 4, 6, and 8; (blood)	None	Wks 2, 4, 6, 8; (blood)	A/PR8 (mo 4)	Day 20; (blood)
Group 2	Aged (13)
Group 3	Young (4)			A/X31 (mo 2)
Group 4	Aged (13)
Group 5	Young (5)	None
Group 6	Aged (10)
Group 7	Young (25)			None
Group 8	Aged (33)
Experiment 2 (Figures 2(a), 2(b), and 2(c))
Group 1	Young (5)	AdC68NP		None	Wk 8; (blood, spleen, and lung)	A/PR8 (mo 4)	Days 5, 20; (blood, spleen, and lung)
Group 2	Aged (8)
Group 3	Young (5)			A/X31 (mo 2)
Group 4	Aged (5)		None
Group 5	Young (5)	None
Group 6	Aged (5)
Group 7	Young (2)			None
Group 8	Aged (4)
Experiment 3 (Figures 3(a) and 3(b))
Group 1	Young (4)	AdC68NP		None		A/PR8 (mo 4)	Day 20; (spleen)
Group 2	Aged (4)
Group 3	Young (4)			A/X31 (mo 2)
Group 4	Young (5)	None	None		None
Group 5	Aged (4)
Group 6	Young (5)			None
Group 7	Aged (3)
Experiment 4 (Figures 4(a), 4(b), 4(c), and 4(e))^1
Group 1	Young (15) [5]	AdC68NP		None		A/PR8 (mo 4)	Checked daily for weight loss and survival; viral titers in lungs tested on day 5; lung infiltrates tested on day 5.
Group 2	Aged (13) [15] [7 ]
Group 3	Young (4) [5]			A/X31 (mo 2)^2
Group 4	Aged (8) [5] [5 ]		None		None
Group 5	Young (5) [5]	None
Group 6	Aged (10) [7] [4 ]
Group 7	Young (25) [9]			None
Group 8	Aged (38) [11] [7 ]

1Number of mice tested for survival are shown in (), numbers of mice tested for viral loads are shown in regular font in [], and numbers of mice used to determine subsets of lung infiltrating cells are shown on bold italic in [].
²Some of the mice were primed at a young age. They were boosted ~18 months later.